Part:BBa_K4811030:Design

TMS(PFOA2)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 80
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Multiple versions of a PFOA-sensitive TMS were attempted (see BBa_K4811024 and BBa_K4811025). In this instance, the aptamer without a stem from J. Park et. al. was included into the D-loop.

Source

The TMS was developed by Avishek Paul during their PhD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.

The PFOA-sensitive aptamer was developed via SELEX , see the publication "Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water", Junyoung Park, Kyung-Ae Yang, Yongju Choi, Jong Kwon Choe, Environment International, 2022, 158 107000."

References

Lorenz, R. and Bernhart, S.H. and Höner zu Siederdissen, C. and Tafer, H. and Flamm, C. and Stadler, P.F. and Hofacker, I.L. "ViennaRNA Package 2.0", Algorithms for Molecular Biology, 6:1 page(s): 26, 2011.